Plants genotoxicity as pollution bioindicator in Jordan using comet assay
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چکیده
منابع مشابه
Transgenic Plants as Sensors of Environmental Pollution Genotoxicity
Rapid technological development is inevitably associated with manyenvironmental problems which primarily include pollution of soil, water and air. In manycases, the presence of contamination is difficult to assess. It is even more difficult toevaluate its potential danger to the environment and humans. Despite the existence ofseveral whole organism-based and cell-based models of sensing polluti...
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Although the Comet assay, a procedure for quantitating DNA damage in mammalian cells, is considered sensitive, it has never been ascertained that its sensitivity is higher than the sensitivity of other genotoxicity assays in mammalian cells. To determine whether the power of the Comet assay to detect a low level of genotoxic potential is superior to those of other genotoxicity assays in mammali...
متن کاملAssessment of Genotoxicity with the Plant Comet Assay
The principle of the comet assay. During electrophoresis, damaged DNA migrates from gelembedded nuclei. Thus, nuclear DNA of individual cells acquires the appearance of a comet, with a head (undamaged DNA) and a tail (damaged DNA). The amount of DNA in the tail correlates with the number of DNA strand breaks and thus indicates the extent of DNA damage. DNA was stained with ethidium bromide and ...
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This paper presents the results of a study on the influence of lead (Pb(+2)) on DNA integrity on plant cells. The study was performed on the root tips of lupin (Lupinus luteus cv. Juno) seedlings treated with two selected concentrations of Pb(NO3)2: 150 and 350 mg l(-1), which were found to inhibit root growth by 50% and 70%, respectively [Rucińska et al. Plant Physiol. Biochem. 37 (1999) 37187...
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The single cell gel electrophoresis (SCGE) assay, also known as the Comet assay, is one of the most promising genotoxicity tests developed in recent years to measure and analyse DNA damage in single cells. The present study was undertaken to assess the in vivo genotoxicity of the synthetic pyrethroid cypermethrin in brain ganglia and anterior mid gut of Drosophila melanogaster. Freshly emerged ...
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ژورنال
عنوان ژورنال: Physiology and Molecular Biology of Plants
سال: 2017
ISSN: 0971-5894,0974-0430
DOI: 10.1007/s12298-017-0491-2